I’m encountering an issue with blob picking for ribosomes in my micrographs. The particles are between 150-250 Å in size, and I’ve selected both circular and elliptical options. However, the algorithm is ignoring a lot of blobs that are clearly visible to the naked eye.
Adjusting the settings in the graph on the left panel allows me to reduce the number of picks, but I can’t seem to make it more inclusive to capture the missed particles. Is there a specific setting or approach I might be overlooking to improve detection?
Tried using a smaller Min. separation distance? It works in concert with the Minimum particle diameter-value to determine how close to allow picks. Could try ~0.4-0.6 depending on how appropriate the minimum diameter value is to begin with.
Agreed re varying min separation distance, I would also highly recommend trying picking on micrographs generated by Micrograph Denoise, we have found that very helpful for small particles, particularly where distinguishing from aggregate is an issue.
