Bin micrographs

Cryo-EM Data Processing
25

Hi Oli and wtempel,

The above issue also taken care of at present (BUT I will revisit it later to provide you more details). I have another problem.

  1. I recently completely a helical reconstruction with resolution up to 3.6Å. Now I want to generate the whole 3.6Å map to a much larger length (it is a phage tail density map). I will need the asymmetric map (at 3.6Å) so that I can symmetrize it to generate a 1200Å long map (the map is ~256Å long). How to obtain the asymmetric map ?

Is there any alternate way to do it ?

  1. I want to sort out heterogeneity based on the Pitch of the helix. I can generate maps for classifications from the asymmetric map, requested above. Following that How to I carry out the classification using the maps in cryosparc ?

Thanks

Andy

How do I do that?