Assistance with Filament Tracer Not Selecting Filaments

Helical Processing
1

Hello cryoSPARC community,

I’ve come across a few posts about issues with the Filament Tracer not selecting filaments, and I seem to be experiencing a similar problem. I’ve tried adjusting several parameters, including keeping the hysteresis low and high thresholds at their default values or reducing them, and testing both template-based and template-free methods.

Unfortunately, as shown in the attached screenshot, the filaments are still being completely ignored.

I would greatly appreciate any suggestions on how to improve this.

Thank you!
Best regards,
Leti

J41_micrograph_j34motioncorrected004611557280259771155_foilhole_24706092_data_24719030_12_20241025_010545_eer_patch_aligned_doseweightedmrc
J41_micrograph_j34motioncorrected004611557280259771155_foilhole_24706092_data_24719030_12_20241025_010545_eer_patch_aligned_doseweightedmrc384×384 181 KB

2

Hello,

Can you add a screenshot of what you tried? And can you show what templates you tried?

Thanks