AssertionError: The following outputs were expected but not produced: alignments3D

Import and Export
1

I am trying to import relion extract .star file into cryoSPARC, I got an error like this.

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Traceback (most recent call last):
File “cryosparc_master/cryosparc_compute/run.py”, line 116, in cryosparc_master.cryosparc_compute.run.main
File “/data/cryosparc-4.6/cryosparc_worker/cryosparc_compute/jobs/imports/run.py”, line 465, in run_import_particles
assert len(validation_errors) == 0, "The following outputs were expected but not produced: " + ", ".join(validation_errors)
AssertionError: The following outputs were expected but not produced: alignments3D

And I want to do 3Dclassfication after importing , but I cannot solve this error!

2

Welcome to the forum @ZA1_Sophia .
Please can you post the output of the command

cryosparcm cli "get_job('P99', 'J199', 'job_type', 'version', 'instance_information', 'status',  'params_spec', 'errors_run')"

where you replace P99, J199 with the import job’s project and job ID’s, respectively.

3

Had the same error. We are on version 4.5.3 and I needed to turn off Particle 3D alignment (alignments3D) in the Strict checking of outputs section for import to work on particles right after extraction.

4

Yeah,I have solved this problem by tranfer relion3.0.8 particles.star file to relion 3.1.3 particles.star file.You can also try it!