My protein has a much more enlongated (50X50X300) and stick-like shape, it looks like a filament but without symmetry. I used the general procedures for particle picking and 2D classification in cryoSPARC. As a result, it indeed showed as a filament-like shape in 2D. However, it seems bad in finding the right center so that a large part of classes look shorter with just centering on one head. No improment when I simply adjusted the mask and re-center threholds.
Do you anyone have some expreriences and suggestions for such a kind of enlongated-shape particles?
Thanks a lot!