Hi @zhenyuanliu,
Thanks for the report. This is indicative of a bug fix that was added in v4.4, fixed with a metadata addition. If you see this message, the behaviour has not changed since v4.3, however it means that the metadata added in v4.4 was not found. This is currently an issue that we’re investigating, and is being discussed in this thread: 2D-classification error of invalid values detected in location/micrographs_psize_A
If you notice obtaining the same AssertionError: Invalid values (zeroes) detected in location/micrograph_psize_A in 2D classification or in remove duplicate particles, please do let us know on that thread and we will contact you about obtaining cs files to help us find the fix.
Best,
Michael