I’ve imported particles from relion 3 into cryosparc v2 to do 2D classification which seems to work so much better in CS than in relion. Then, I exported the particles back into relion using pyem to do 3D refinement in relion to compare to my CS results (which don’t look super great).
Using the particles in 2D classification, in Initial model and 3D classification works perfectly fine. But the 3D refinement immediately fails and I can’t find out why.
Has anyone experienced this or has any clue why this may be? I have attached the error that I get and the relion parameters I used. I tried various relion parameters by now but I just can’t figure it out… I also tested my parameters with “relion-only” particles and with them, I could get it to work. It might be something stupid… XD
Any help is greatly appreciated!