Dear cryosparc team,
We have two datasets imported separately in the same project in version 4.6.2 and have a refined structure. The metadata xml files were not imported. We were asked by a reviewer to look at higher order corrections. Is there a way to add these back without having to start the processing from scratch.
Would case #2 of the tutorial work for us? Tutorial: EPU AFIS Beam Shift Import | CryoSPARC Guide
Best,
Joe
Case 2 would work for you.
Import beam shifts with the “Import Beam Shifts” job, then apply correct grouping with the “Exposure Groups” job.
If you toggle the option to “Correspond particles to exposures and enforce consistency of exposure group IDs” you can run Global CTF refinement with the expanded groups from your last processing step (e.g. non-uniform refinement) then run another NU refine.
Great - will do this then many thanks for the quick reply. Would you refine the different corrections separately or together?
As a follow up. I should apply case 2 to each imported movie stack. Further, I would imagine these initial imports would be assigned different exposure groups (0 and 1). Does cryosparc automatically take this into account when assigning IDs? Would i need to modify the combine strategy.
For example, if dataset A has 60 exposure groups and dataset B 40. I should end up with 100 exposure groups numbered 0-99 (these grids were loaded randomly with respect to each other). Or do I need to offset the starting number for the second exposure group utilities job by 60.
Yes, you’ll need to offset the exposure group start for each import group, or they will overlap.