I have a doubt if i convert .cs extracted file from cryosparc extraction job and soft link the for example extraction job to the relion directory along with that converted star file and following that if i used that star file for 2D classification → select classes–> followed by select_particles.star importing in cryosparc by connecting the relavent exposures.
Now if i a follow this and try to rextract the select particles in cryosparc should also then i have to use --inverty for rextraction . This doubt is coming because in the relion for classification is again using the .mrcs which are generated in cryosparc only but i guess coordinate is already inverted for relion’s feasibily ?? is my doubt correct in this conversion if yes then should i use --inverty during cryosparc conversion @DanielAsarnow