Ab-initio yield better map than NU-refinment on a small membrane protein complex

3D Refinement
2

  • For very small proteins, NU-refine can be sensitive to the initial lowpass, and the default can be a bit aggressive. Reducing to 15 or even 12Å may give better results, if the initial ab initio model is already pretty good

  • Try proceeding directly to local refinement (with NU regularization on) from the orientations in the ab initio job.

  • Try NU-refine with a static (and very soft) mask, rather than using the default dynamic masking parameters.

5 Likes