Ab initio reconstruction unused particles

[wtempel]

@ldlamini

Does anyone know what causes this

In the single-class case specifically, an ab initio 3D reconstruction job may not “consider” all particles. This is the expected behavior of the job.

3D ab initio job does not load entire particle stack

@olibclarke It’s not so much a rejection of particles, but an “omission from consideration” for single class ab initio reconstruction, where the total number of iterations to run is determined differently from the multi-class case.

@ldlamini

how to fix it?

@wtempel

After single-class ab initio reconstruction, one can still include all particles in a subsequent job by connecting as inputs

• all particles input to ab initio reconstruction
• the volume output by ab initio reconstruction