There is a very bright spot in my 2D image.It dosenot like a protein dnesity.
it DOES look like a protein density to me. and the rest is micelle. class 1 is not aligned well but class 2,8,10,13,14 tells a compelling story of a small membrane protein.
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Agreed, looks like protein to me
ok,thanks! but this one is not my target protein, it is too small.
I see. thanks. I don not know the protein density can be so bright.