Hi @rbs_sci, sorry for the delay getting back to you on this. I have a theory regarding a possible cause… In 4.5 we introduced particle recentering and it’s also on by default. Are you using it? If so, one possible cause is particle recentering followed by rejection of particles too close to a micrograph edge. Those steps are done after the initial screening for micrographs with less than 2 particles. That ordering is definitely a bug, but I don’t know if it’s your bug. Are you using particle recentering? If so, can you isolate the problematic movie and see if running with recentering off fixes the issue?
@qchen tagging you in case the same workaround works for you as well.