In the 3D flex pre-print, in several figures the authors display convected volumes at +1 and -1 standard deviations in the latent space. How would I do this for my own figures? Does the 3D flex generator job automatically output a volume series from +1 to -1 standard deviations? In that case I assume I just use the first and last frames for comparison.
Cheers! 
Hi @scottgardner,
In the 3D Flex generator job, the latents vary by default from the 2nd to the 98th percentile for each dimension. To instead run the generator but querying latent variables from -1 to +1 standard deviations, the best approach would be by using CryoSPARC Tools and following the " 3D Flex: Custom Latent Trajectory" example, which shows how this may be done for a more advanced custom trajectory. In your case, you wouldn’t need the interactive component to that notebook; it would be sufficient to calculate the standard deviation of the latent z values for each dimension, and then output a linearly spaced sampling in the same fashion as shown by the notebook.
The notebook shows how to output these custom latents to a CryoSPARC workspace as an External Job. These latents can then be passed into the flex generator job to actually create the volumes at each latent coordinate
Hope this helps,
Michael
Awesome, thanks a lot!