As new entry into EM I have many questions which some of you may find dumb. Sorry to waste your time with such questions. I found this platform is the best EM teacher.
I did up to 2D classification in relion. Then I imported particles into cryosparc using cryosparc import option and selecting particle metapath.
Simultaneously, I also imported the motion corrected micrographs into cryosparc. Did the CTF correction in cryosparc and extracted the particles using the imported particles file.
Then I ran a set of 2D classification. Selected good particles and ran the Ab intio 3D. It gave me a reasonable map. Then I did nonuniform refinement. Map was looking decent.
Now my question is can I use this xxxvolume.mrc as reference and do the 3D classification in relion using the same set of particles which I first imported into cryosparc? Or do I need to export this map and particles into relion again.