@apunjani @olibclarke I see more and more occasions where Reviewers ask to try to resolve flexibility of a region by using other approaches than cryoSPARC, i.e. 3D classification in Relion without alignment. Is there any ratio behind such idea, and is there any reason to believe that such approach will perform better than local refinement, particle subtraction, 3D-variability analysis, etc, in cryoSPARC. Is there any algorithm and parameters in cryoSPARC that would do the same as 3D classification without alignment in Relion ? That would be so much easier than exporting cs files and importing in Relion, with all the troubles encountered in this approach.
I would very much appreciate if you could give me your opinion on this !
Many thanks !
Jacopo