Hi，
As a new user of Cryosparc, I attempted to resolve a protein DNA complex structure with CryoEM. For now, after initial blob pick up and 2D classification, i thought I got good template. The predicted structure and the template as blow.
However, after using the template for picking up particle, the results was strange, lot of garbages were picked.
Do you have any advice for how to get better 2D classification resluts?
Thanks!