I’m working with a dataset that contain a main particle (70 kDa) bound to two satellite particles (30 kDa each). Using ‘standard’ processing, I was able to solve the structure of the main particle at 3.5A. The satellite particles are not-visible in any step of the processing, but can be seem in the extracted particles
I would like to show that they exist at least in 2D classification. For that, I extracted the particles using larger boxes and tried to 2DC those modifying parameters like Max res, solvent clamp, uncertainty factor, etc. The results I have so far are not convincing
any suggestion of how I configure my 2DC for enhance visualization of these particles?