2D classification counts all particles as duplicates after reextraction and deletion of original extraction

Particle Picking
2

Hi there @kbjoernsson,

Thanks for the post. The suggested procedure for handling this issue, for CryoSPARC v4.4.1, has been described here. In short: Remove duplicate particles can be done via the standalone job with a specified micrograph pixel size, and the remove duplicates parameter in the 2D classification job can be disabled.

The reason for this was due to a bug that existed for particle datasets picked before version v4.4.1, in which not all metadata including the micrograph pixel size was tracked.

Let me know if this answers your question
Michael

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