Hi @spunjani, is there a detailed workflow described anywhere in terms of how to use volume alignment tools to recenter and re-extract in conjunction with signal subtraction, especially in a context where one wants the subtracted particles in a smaller box?
I guess it would be:
1: Use Volume Alignment tools to recenter on region to be kept.
2: Re-extract in smaller box around region of interest
2: Homogeneous reconstruction in smaller box
3: Particle subtraction with soft mask around everything to be subtracted
Is that more or less it?
Cheers
Oli