Hi @leetleyang, are your movies mounted on a network filesystem of some sort? A while ago we noticed that reading TIFFs and EERs over a network was extremely slow for some users and we implemented a workaround involving copying the file into shared memory and reading it from there. Unfortunately, that workaround didn’t play nicely with reference based motion correction, so we disabled it in RBMC jobs. If you’re on a network filesystem of some sort, I expect this is the problem that you’re experiencing.
It’s on our radar to fix this, and while I can’t be too specific on a timeline, a future release of CryoSPARC won’t suffer from this performance anomaly. In the meantime, here’s one possible workaround: if you have enough space on your cache SSD or any locally attached storage, you could copy the movies onto a local SSD and read them from there.