Hello everyone, thank you for providing information and insight here.
Last week we released a patch 210817 for cryoSPARC v3.2 which includes a flag to optionally enable our new the new in-memory TIFF reading system. Installation instructions here (you’ll have to restart cryoSPARC after applying). This will also be available in the next full cryoSPARC release.
To enable the new strategy, add the following line to cryosparc_worker/config.sh (note: NOT to cryosparc_master):
export CRYOSPARC_TIFF_IO_SHM=true
Please post here if you run into further issues with this.