Hi,
I collected a dataset on Krios/K2/Super-res (0.55A/pix, 40 frames). The micrographs were collected on EPU - non-gain corrected, normalized 4-bit encryption. Each file was 1.5GB and would not load into cryosparc directly. To load them into crysosparc, I used: newstack -by 0 command. The resultant files were 1.2GB. This did not make sense to me why the file size would be smaller. I however loaded them into cry0sparc and did patch motion correction and the results were HORRENDOES…
I am wondering if the 4-bit to 8-bit conversion messed up anything. It does not make sense to me why the file size would become smaller. Also, I tried to convert them to 16-bit noted by @stavros in this thread (using the newstack -mo 1 option) and the resultant file size was 1.8GB - again this does not make sense, since I would assume it to be 2.4GB (2x size of the file generated by newstack -by 0)
Do you have any kneww-jerk thoughts or suggestions? I would really appreciate it if you could help me troubleshoot this.
Thank you