I see @Itai, thanks for that additional information! If you have particle positions which correspond to A
points, you may be able to use cryosparc-tools to find B
positions. Here’s the general process I’d use, applying the below steps to each micrograph:
- Load in all particle positions
- Filter out just the
A
subset. - For each
A
particle:
a. Add and subtract the A/B offset distance fromfilament/position_A
. This is the position of the particle along the filament contour, in Å. For example, if the filament is 50 Å long and the particle was halfway along the filament, this value would be25
. Note that this does take into account filament curvature.
b. Find particles which are within some small distance from the offsetfilament/position_A
along the filament, if any. These would be your candidateB
particles.
An explanation of all of the filament parameters is available here.
I hope that is helpful! Please let me know if there’s anything else I can clear up for you!