If I re-extract (with a bigger box and no bin) after doing both global and local CTF refinement, ¿is the refined CTF information kept in the reextracted particles?
Yes, it is, unless you deliberately switch it off (there is an option to force re-extract CTF params from mics)
1 Like
I did symmetry expansion (C4) and then downsampled the symmetry expanded particles for 3D classification with focused mask on one subunit. Now I want to use the original symmetry expanded particles for local refinement. Should I use the particle blob from the symmetry expansion job, instead of the original particle extraction job?
Yes. (post must be at least 20 characters)
Great.
Thank you so much.