Can't extract from older data

Cryo-EM Data Processing
1

hi

I have been working on a project for quite a while. It has gone through several upgrades in cryosparc so it’s probably expected I’ll get weird errors when revisiting.

I am trying to re-extract my particles from their original micrographs. I get an error something about negative stain? This is cryo data, there is no stain data mixed in. No idea what this means.

Traceback (most recent call last): 
File "cryosparc2_worker/cryosparc2_compute/run.py", line 85, in cryosparc2_compute.run.main 
File "cryosparc2_compute/jobs/extract/run.py", line 129, in run_extract_micrographs_multi 
    negative_stain = micrographs_dset.data['mscope_params/neg_stain'][0] 
KeyError: 'mscope_params/neg_stain'
2

HI @orangeboomerang,

This is most likely because of the data being processed by an older version of cryoSPARC. See the following post for a solution: `mscope_params/neg_stain` error message