Breathing Effect of (AB)n-type protein assemblies

[olibclarke]

Hi Yufeng

You might want to read this:

Case Study: End-to-end processing of encapsulated ferritin (EMPIAR-10716)

We’re excited to be sharing a case study on the end-to-end processing of encapsulated ferritin in CryoSPARC! This case study demonstrates some advanced tools and processes that enable processing of a structure with unconventional symmetry and geometry characteristics. Topics covered include: Ab-Initio Reconstruction of high-symmetry datasets handling local symmetry and non-point-group symmetry use of Volume Alignment Tools and Align 3D Maps for custom geometry operations jobs such as 3D Classi…

Which addresses exploiting non-point group symmetry in a pseudo-symmetry expansion approach, which may be something worth trying for your data.

Cheers
Oli