Assess the picking and the 2D classes quality for small, low SNR particles

[CryoEM2]

Topaz extract throws error when more micrographs are added

they look great. you can use that trained model to “topaz extract” = pick particles on any number of micrographs. Best performance using it to pick those in the same dataset. The number of particles it picks will strongly depend on the expected number of particles you provided in the training. If you are looking for a rare particle, you may still want to provide a number of expected particles close to the full particle set per micrograph to get every rare particle, but if the number reflects…

You can train on a subset of micrographs and “topaz extract” on full set. Use very clean particle set.