Ab-initio has 90% unused particles

[stjiafle]

Hi wtempel,
Thank you for pointing out this thread since I missed it from my initial search.
Similar to them, I have my input as

[CPU:  191.7 MB]
Importing job module for job type homo_abinit...

[CPU:  293.7 MB]
Job ready to run

[CPU:  293.7 MB]
***************************************************************

[CPU:  729.9 MB]
Using random seed for sgd of 1044547418

[CPU:  814.5 MB]
Loading a ParticleStack with 790250 items...

I already did this step as described by jenchem:

@jenchem

Looking back, it appears I loaded all of the particles from all stacks (ie all particles that I wanted for further processing) into one 2D-classification job, then selected all of the output from that one 2D-classification job to start a 3D ab initio. That seems to have worked for my case.

My dataset come from two different import movies, but it combined into one Patch CTF, and one template picker, extract mics, and 2D class.

I tried doing homogeneous refinement using the unused particles and it doesn’t go so hot.
So right now I’m importing this job to the other computer (which currently using cryosparc v3.9) and try over there and see if the problem still persist.