Resolution in FSC

request_recorded

#1

Hi, I am getting “abnormally high” resolution in 2.4.2 version from FSC curves (see attached) Selection_195|690x330. It looks to me like the programs tries to find intersection of FSC with 0.143 threshold going from high resolution end, not from zero. Is that true?
Thanks,
Michael


#2

Hi @mbs,

Can you reattach your image? It doesn’t seem to be working.


#3

Continuing the discussion from Resolution in FSC:

Hi @sarulthasan,
sorry for that, i hope this time it would be visible.
Michael


#4

Yes - cryoSPARC calculates res as the last time the FSC crosses 0.143, rather than the first, I’ve reported this as well, it is evidently a bug, hopefully fixed soon


#5

I agree, and that is causing problems with refinement.


#6

Hi all,

This issue will be fixed in an upcoming release.

Best,
Ali


#7

Thanks you Ali,
Could you send me the link to beta version?
all the best,
Michael


#8

Hi @mbs, please check your inbox for a direct message from me!

Best,
Ali