Hi,
I am using the Cryosparc v3.01 to process a D3 complex, but the refinement cannot find the correct D3 axes. It is right when using C3 symmetry, but will be messed up when switching from C3 to D3. I tried to check or uncheck “Do symmetry alignment”, but all failed. Any suggestion would be greatly appreciated!
It’s still not right if I input the previous C3 map (which is also a C2 map) and impose a C2 symmetry during refinement, and it will generate a D2 map instead of a D3 map, with the previous C3 axis replaced by a C2 axis on the Z-axis.