I have been trying to build an ab initio model using Cryoparc, but all of the reconstructions are essentially hollow spheres or similar shapes lacking a central density.
This data set has gone through several rounds of 2D classification in Relion and gives very nice 2D class averages.
A few notes:
- this is phase-shifted data
- the particles are small (~200 kDa)
- the particles are quite densely packed, with multiple neighboring particles visible.
I’ve tried playing around with the mask dimensions, but with no luck.
Any suggestions would be helpful!