I have few questions for you, I’m a newbie using cryosparc
first of all, How to decide when to use CTFFind or CTF? I can’t find anything on the internet… I’ve always used CTFFind with micrographs, but is it better to use CTF multi when it comes to movies?
secondly, I have to do the structure of a protein. Its cryoEM, TitanKryos.
When it come to motion correction, I obtained something like this
I dont really know what’s wrong, or, if this is wrong… I know that in a perfect case we have a certain scheme, but here, I dont know what to think.
after that, must I do a CTFFind or CTF ?
Sorry for all these questions (and I have still a lot, but if someone can anwsear to these its pretty nice)
have a good day