Local symmetry operators for refinement



We have an asymmetric particle containing multiple copies of the same sub-complex. Can we impose local symmetry during refinement in cryosparc similar to what has been implemented in relion? If not, is this on the to-do list for future upgrades?


Hi @jochen,

We are currently working on implementing support for non-standard symmetry in cryoSPARC. The first case we are working on is supporting multiple copies of an identical subvolume located at arbitrary locations on the volume, e.g. non-crystallographic symmetry. The second case will allow for the local refinement of symmetric units that are flexible with respect to one another.