I/O error during Homogeneous Refinement after re-extracting unbinned particles

3D Refinement
2

@chaoyang May I ask:

  1. What version of CryoSPARC do you use.
  2. Have you tried, as a diagnostic step, to run homogeneous refinement with the Cache particle images on SSD parameter disabled?
  3. What is the output of the command
    df -hT /home/u18601538/software/cryosparc/cache
  4. Is the filesystem where /home/u18601538/software/cryosparc/cache significantly faster than the filesystem where CryoSPARC projects are stored?

As an aside: Because caching tends to entail the frequent writing of large amounts of data, the wear on underlying storage devices can be significant. Device wear may lead to failure of the storage, which, in case of cached data, is a minor problem because a primary copy of the data may still exist (in the project directory), unless the primary copy stored on the same device as the cache. For this reason, one may want to store the cache on separate devices from more valuable data, like raw movies, CryoSPARC project directories, or the CryoSPARC database.

Gentle reminder: Please post error messages as text instead of as a screenshot to enable users with a similar problem to more easily find this discussion.