In Cryosparc CTF refinement tutorial for Nonuniform Refinement, it mentions:
On-the-fly CTF refinement cannot be done during a
Non-uniform Refinement, so particles should be processed through the standalone
Local CTF Refinementthen
Global CTF Refinementjobs first.
I am wondering if I did
Optimize per-particle defocus and
Optimize per-group CTF params on-the-fly in Homogeneous Refinement (NEW!)) already, can I just use those particles from Homogeneous Refinement to do Non-uniform Refinement with Enable higher-order CTF on? Or still do I have to do standalone
Local CTF Refinement then
Global CTF Refinement jobs first?