If the input volume changes, I think the result in the Global CTF Refinement will change. Is this the right understanding? According to the explanation, the Global CTF Refinement gets “high-order” information from the density map that cannot be analyzed at the microscope level, so I made an analogy like this. Is there something I misunderstood?
Whenever I do a refinement after classification, the volume makes subtle but precise changes, so I’m wondering if it’s appropriate to do Global CTF refinement for each classified particle at this time.