Hi, all
I’ve run heterogeneous refinement with a refinement box size of 240 voxels.
After the heterogenous refinement, I want to run an NU refinement.
My question is, do I need to re-extract the particles using the original box size (380) to do the following refinement?
And how?
Using all of the original micrographs and the particles classified by heterogeneous refinement?
Thanks.