Built-in HAIR half-maps with opposite handedness

3D Reconstruction
1

Hello all,

I am using the built-in Homogeneous Ab-Initio Refinement job for the first time. It appears that my resulting half-maps have different handedness. Does this negatively influence downstream Homogeneous Reconstruction or Local Refinement jobs? My reconstruction and refinement jobs have given me poor results thus far, and I am unsure if the handedness is the issue, or something else. Running HR-HAIR as described in Clarke’s preprint has worked amazingly for me in the past, but I am struggling to get the comparable results from the CryoSPARC implementation.

Thanks!

2

Yes if the half maps have different hands that will definitely cause problems downstream!

You can run the half jobs separately and then manually align & generate the half sets, it requires a small amount of scripting but is fairly straightforward