Dear cryoSPARC team
I have a question about the 3D sorting/classification method in cryoSparc.
So now I have good reconstructions for 3 different helical volumes: they have a subtle difference in diameter (let’s say 250Å vs 255 Å vs 260Å) and they have different helical symmetries. I have a particle subset that contains all 3 classes and I want to do reference-based soring using this subset. Can this be done in “Heterogeneous Refinement”? Ideally, I want to keep the volumes at a high resolution ~4Å for this sorting and assign all the particles into those 3 classes in 1 iteration. Is this possible?